calibrate_per_cell_total_proposal: Calibrate_per_cell_total_proposal

In monocle: Clustering, differential expression, and trajectory analysis for single- cell RNA-Seq

Description

Calibrate_per_cell_total_proposal

Usage

calibrate_per_cell_total_proposal(relative_exprs_matrix, t_estimate,
  expected_capture_rate, method = c("num_genes", "tpm_fraction"))

Arguments

relative_exprs_matrix	The matrix of relative TPM expression values
t_estimate	the TPM value that corresponds to 1 cDNA copy per cell
expected_capture_rate	The fraction of mRNAs captured as cDNAs
method	the formula to estimate the total mRNAs (num_genes corresponds to the second formula while tpm_fraction corresponds to the first formula, see the anouncement on Trapnell lab website for the Census paper)

monocle documentation built on Nov. 8, 2020, 5:06 p.m.