inst/tests/test.reduceDimension.R

In monocle: Clustering, differential expression, and trajectory analysis for single- cell RNA-Seq

library(monocle)
library(HSMMSingleCell)
context("reduceDimension is functioning properly")

pd <- new("AnnotatedDataFrame", data = HSMM_sample_sheet)
fd <- new("AnnotatedDataFrame", data = HSMM_gene_annotation)
HSMM <- newCellDataSet(as.matrix(HSMM_expr_matrix), phenoData = pd, featureData = fd)

HSMM <- newCellDataSet(as(umi_matrix, "sparseMatrix"),
                       phenoData = pd,
                       featureData = fd,
                       lowerDetectionLimit = 0.5,
                       expressionFamily = negbinomial.size())