BASiCSSimulate: BASiCS simulation

In splatter: Simple Simulation of Single-cell RNA Sequencing Data

Description

Simulate counts using the BASiCS method.

Usage

BASiCSSimulate(
  params = newBASiCSParams(),
  sparsify = TRUE,
  verbose = TRUE,
  ...
)

Arguments

params	BASiCSParams object containing simulation parameters.
sparsify	logical. Whether to automatically convert assays to sparse matrices if there will be a size reduction.
verbose	logical. Whether to print progress messages
...	any additional parameter settings to override what is provided in params.

Details

This function is just a wrapper around BASiCS_Sim that takes a BASiCSParams, runs the simulation then converts the output to a SingleCellExperiment object. See BASiCS_Sim for more details of how the simulation works.

Value

SingleCellExperiment containing simulated counts

References

Vallejos CA, Marioni JC, Richardson S. BASiCS: Bayesian Analysis of Single-Cell Sequencing data. PLoS Computational Biology (2015).

Paper: 10.1371/journal.pcbi.1004333

Code: https://github.com/catavallejos/BASiCS

Examples

if (requireNamespace("BASiCS", quietly = TRUE)) {
    sim <- BASiCSSimulate()
}

Example output

Loading required package: scater
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, basename, cbind, colMeans, colSums, colnames,
    dirname, do.call, duplicated, eval, evalq, get, grep, grepl,
    intersect, is.unsorted, lapply, lengths, mapply, match, mget,
    order, paste, pmax, pmax.int, pmin, pmin.int, rank, rbind,
    rowMeans, rowSums, rownames, sapply, setdiff, sort, table, tapply,
    union, unique, unsplit, which, which.max, which.min

Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: ggplot2

Attaching package: 'scater'

The following object is masked from 'package:stats':

    filter

Warning messages:
1: no function found corresponding to methods exports from 'DelayedArray' for: 'acbind', 'arbind' 
2: no function found corresponding to methods exports from 'SummarizedExperiment' for: 'acbind', 'arbind' 

splatter documentation built on Dec. 3, 2020, 2:01 a.m.