inst/MFIaggr.gui/readme.md

In chipPCR: Toolkit of Helper Functions to Pre-Process Amplification Data

About

MFIaggr is used for a fast multiple comparison of the cycle dependent variance of the fluorescence. It is a web-based implementation of function from package chipPCR.

Authors: Stefan Roediger, Michal Burdukiewicz.

Readme

1. Choose file - a .csv file with a fluorescence data and cycle number.
2. Header - must be checked if input table has header.
3. Type of .csv file - choose type of .csv file (depending on decimal separator).
4. Column containing the cycle data - number of column containing the cycle number.
5. Relative standard deviation - show relative standard deviation in percent.
6. Median and MAD - must be checked if median and MAD should be used instead of mean and standard deviation.
7. Lower and upper border of the region of interest - two cycle numbers defining upper and lower limit of the region of interest (ROI) used for the density and quantile plot.

chipPCR documentation built on March 5, 2021, 9:06 a.m.