RPA: Recombinase polymerase amplification (RPA) by Lutz et...

In chipPCR: Toolkit of Helper Functions to Pre-Process Amplification Data

Description

Real-time amplification plot of an Recombinase Polymerase Amplification (RPA) by Lutz et al.(2009) in a centrifugal microfluidic foil cartridge.

Usage

data("RPA")

Format

A data frame with 184 observations on the following 2 variables.

Reaction.Time

Reaction Time [min]

RFU

Relative fluorescence units [RFU]

Details

The data were digitized as described by Poisot (2011). The image for data extraction was taken from Figure 3b by Lutz et al.(2009). The amplification curve present the results of a cross-contamination test of a foil disk for a sample containing 2000 copies of the mecA gene. For further experimental and technical details refer to Lutz et al.(2009).

Source

The original data (open access under CC BY-NC-ND license) were taken from Figure 3b by Lutz et al.(2009).

References

Poisot, T. (2011). The digitize Package: Extracting Numerical Data from Scatterplots. The R Journal 3, 25–26.

Lutz, S., Weber, P., Focke, M., Faltin, B., Roth, G., Piepenburg, O., Armes, N., Mark, D., Zengerle, R., and von Stetten, F. (2009). Isothermal Polymerase Amplification in a Centrifugal Microfluidic Foil Cartridge. Procedia Chemistry 1, 529–531.

Examples

data(RPA)
plot(RPA, main = "RPA by Lutz et al.(2009)", xlab = "Reaction Time [min]", 
     ylab = "Relative fluorescence units [RFU]", type = "l")
abline(h = 5, lty = 2, col = "grey")

chipPCR documentation built on March 5, 2021, 9:06 a.m.