BridgeRHKGenes: Classic BRIC-seq data analysis pipeline

Description Usage Arguments Details Value Note Author(s) References Examples

View source: R/Z0_BridgeR_core.r

Description

RNA half-life for each gene is calculated using the RPKM of house-keeping genes.

Usage

 1
 2
 3
 4
 5
 6
 7
 8
 9
10
11
12
13
BridgeRHKGenes(InputFiles,
                           InforColumn=4, 
                           InforHKGenes=2, 
                           HKGenes=c("GAPDH","PGK1","PPIA","ENO1","ATP5B","ALDOA"), 
                           group, 
                           hour, 
                           RPKMcutoff=0.1,
                           CutoffDataPointNumber = 4,
                           CutoffDataPoint1 = c(1,2),
                           CutoffDataPoint2 = c(8,12),
                           ThresholdHalfLife = c(8,12),
                           CutoffRelExp=0.001,
                           ModelMode="R2_selection")

Arguments

InputFiles

Vector

InforColumn

Integer

InforHKGenes

Integer

HKGenes

String(gene_name)

group

Vector(string)

hour

Vector(number)

RPKMcutoff

Float

CutoffDataPointNumber

Integer

CutoffDataPoint1

Vector(Integer or float)

CutoffDataPoint2

Vector(Integer or float)

ThresholdHalfLife

Vector(Integer or float)

CutoffRelExp

Float

ModelMode

Bool(True or False)

Details

RNA half-life for each gene is calculated using the RPKM of house-keeping genes.

Value

text files and fig files

Note

2015-11-05

Author(s)

Naoto Imamachi

References

https://github.com/Naoto-Imamachi/BRIC-seq_data_analysis/tree/master/BridgeR

Examples

 1
 2
 3
 4
 5
 6
 7
 8
 9
10
11
12
13
14
15
16
17
18
19
20
##---- Should be DIRECTLY executable !! ----
##-- ==>  Define data, use random,
##--	or do  help(data=index)  for the standard data sets.

## The function is currently defined as
function (InputFiles,
                           InforColumn=4, 
                           InforHKGenes=2, 
                           HKGenes=c("GAPDH","PGK1","PPIA","ENO1","ATP5B","ALDOA"), 
                           group, 
                           hour, 
                           RPKMcutoff=0.1,
                           CutoffDataPointNumber = 4,
                           CutoffDataPoint1 = c(1,2),
                           CutoffDataPoint2 = c(8,12),
                           ThresholdHalfLife = c(8,12),
                           CutoffRelExp=0.001,
                           ModelMode="R2_selection") 
{
}

Naoto-Imamachi/BridgeR documentation built on May 7, 2019, 6:05 p.m.