CreateSeuratObjectList: Create a list of Seurat Objects

In jacobheng/cellwrangler: Supplemental toolkit for single-cell RNAseq analysis

Description

CreateSeuratObjectList() creates a list of Seurat objects for use in the RunMultiCCA function in the R package Seurat. The function splits a single aggregated barcoded expression matrix into separate matries based on the suffix at the end of each cellular barcode e.g. ("ACTAGGAGACAGGTGC-1").

Usage

CreateSeuratObjectList(exprs_matrix, phenoData, scaleData = FALSE)

Arguments

exprs_matrix	an expression matrix; row names must be gene names and column names must be cell barcodes
phenoData	dataframe where rows are cells and rownames are cell barcodes, columns are fields defining cellular characteristics. Row names of phenoData must match column names of exprs_matrix.

Value

A list of Seurat Objects

Examples

list <- CreateSeuratObjectList(exprs_matrix = exprs(dat), phenoData = pData(dat))

jacobheng/cellwrangler documentation built on Aug. 12, 2019, 6:49 a.m.