ISS_rotate: Rotate and flip ISS data

In mashranga/MolDia: Single cell In-Situ sequencing (ISS) data analysis

Description

Rotate by angle and flip by axix of ISS data

Usage

ISS_rotate(data, rot = 0, flipby = NULL)

Arguments

data	Input data in class MolDiaISS. Output of readISS.
rot	Rotation angle (Degree). Default is zero. Negative angle define the anti-clock wise rotation.
flipby	Flip the data points by x or y axis. Default is NULL. Possible value is NULL, "x", "y".

Examples

# Read ISS data
mydata  <- readISS(file = system.file("extdata", "Hypocampus_left.csv", package="MolDia"),
                   cellid = "CellId", centX = "centroid_x", centY = "centroid_y")

# Flip/rotate ISS data
mydata_x  <- ISS_rotate (data = mydata, rot = 0, flipby = "x")
mydata_y  <- ISS_rotate (data = mydata, rot = 0, flipby = "y")
mydata_90 <- ISS_rotate (data = mydata, rot = 90)

# Plot ISS data
res     <- ISS_map(data = mydata, what = "cell", main = "Left hippocampus")
res1    <- ISS_map(data = mydata_x, what = "cell", main = "Left hippocampus")
res1    <- ISS_map(data = mydata_y, what = "cell", main = "Left hippocampus")
res1    <- ISS_map(data = mydata_90, what = "cell", main = "Left hippocampus")

mashranga/MolDia documentation built on May 26, 2019, 9:36 a.m.