Description Usage Arguments Details Value Author(s) References Examples
View source: R/computePWMScore.R
computePWMScore
will compute and extract all sites that exhibit a
PWM Score higher than a threshold.
This threshold (see PWMThreshold
) will determine the percentage
of total sites that should NOT be considered.
1 2 | computePWMScore(genomicProfiles,DNASequenceSet,
loci = NULL, chromatinState = NULL,parameterOptions=NULL,cores=1, verbose = TRUE)
|
DNASequenceSet |
|
genomicProfiles |
|
loci |
|
parameterOptions |
|
chromatinState |
|
cores |
|
verbose |
|
After determining genome wide scores, it is possible to only compute and
extract high affinity sites (in the sense that they have a high PWM Score).
If a PWMThreshold
is not set by user,
the default value is set at 0.7.
This means that 70 % of sites will NOT be selected.
Only the top 30 % will be computed and extracted.
If one is interested in all PWM Scores at a genome wide scale
( or accessible DNA ), this is possible by setting
PWMThreshold
to zero.
computePWMScore
will return a
genomicProfiles
object.
The profiles
slot will have been updated.
This slot will now contain a GRangesList
with each element being a GRanges
.
This GRanges will contain postion of each sites
(start, end and strand) and the PWMScore associated to that site.
Patrick C.N. Martin <pm16057@essex.ac.uk>
Zabet NR, Adryan B (2015) Estimating binding properties of transcription factors from genome-wide binding profiles. Nucleic Acids Res., 43, 84<e2><80><93>94.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 | #Data extraction
data(ChIPanalyserData)
# path to Position Frequency Matrix
PFM <- file.path(system.file("extdata",package="ChIPanalyser"),"BCDSlx.pfm")
#As an example of genome, this example will run on the Drosophila genome
if(!require("BSgenome.Dmelanogaster.UCSC.dm3", character.only = TRUE)){
if (!requireNamespace("BiocManager", quietly=TRUE))
install.packages("BiocManager")
BiocManager::install("BSgenome.Dmelanogaster.UCSC.dm3")
}
library(BSgenome.Dmelanogaster.UCSC.dm3)
DNASequenceSet <- getSeq(BSgenome.Dmelanogaster.UCSC.dm3)
eveLocusChip<-processingChIP(eveLocusChip,eveLocus)
#Building data objects
GPP <- genomicProfiles(PFM=PFM,BPFrequency=DNASequenceSet)
# Computing Genome Wide
GenomeWide <- computeGenomeWideScores(DNASequenceSet = DNASequenceSet,
genomicProfiles = GPP)
#Compute PWM Scores
PWMScores <- computePWMScore(DNASequenceSet = DNASequenceSet,
genomicProfiles = GenomeWide,
loci = eveLocusChip, chromatinState = Access)
PWMScores
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