Description Usage Arguments Details Value

Monocle aims to learn how cells transition through a biological program of
gene expression changes in an experiment. Each cell can be viewed as a point
in a high-dimensional space, where each dimension describes the expression of
a different gene in the genome. Identifying the program of gene expression
changes is equivalent to learning a *trajectory* that the cells follow
through this space. However, the more dimensions there are in the analysis,
the harder the trajectory is to learn. Fortunately, many genes typically
co-vary with one another, and so the dimensionality of the data can be
reduced with a wide variety of different algorithms. Monocle provides two
different algorithms for dimensionality reduction via `reduceDimension`

.
Both take a CellDataSet object and a number of dimensions allowed for the
reduced space. You can also provide a model formula indicating some variables
(e.g. batch ID or other technical factors) to "subtract" from the data so it
doesn't contribute to the trajectory.

1 2 3 4 5 | ```
reduceDimension(cds, max_components = 2, reduction_method = c("DDRTree",
"ICA", "tSNE", "SimplePPT", "L1-graph", "SGL-tree"), norm_method = c("log",
"vstExprs", "none"), residualModelFormulaStr = NULL, pseudo_expr = 1,
relative_expr = TRUE, auto_param_selection = TRUE, verbose = FALSE,
scaling = TRUE, ...)
``` |

`cds` |
the CellDataSet upon which to perform this operation |

`max_components` |
the dimensionality of the reduced space |

`reduction_method` |
A character string specifying the algorithm to use for dimensionality reduction. |

`norm_method` |
Determines how to transform expression values prior to reducing dimensionality |

`residualModelFormulaStr` |
A model formula specifying the effects to subtract from the data before clustering. |

`pseudo_expr` |
amount to increase expression values before dimensionality reduction |

`relative_expr` |
When this argument is set to TRUE (default), we intend to convert the expression into a relative expression. |

`auto_param_selection` |
when this argument is set to TRUE (default), it will automatically calculate the proper value for the ncenter (number of centroids) parameters which will be passed into DDRTree call. |

`verbose` |
Whether to emit verbose output during dimensionality reduction |

`scaling` |
When this argument is set to TRUE (default), it will scale each gene before running trajectory reconstruction. |

`...` |
additional arguments to pass to the dimensionality reduction function |

You can choose two different reduction algorithms: Independent Component
Analysis (ICA) and Discriminative Dimensionality Reduction with Trees (DDRTree).
The choice impacts numerous downstream analysis steps, including `orderCells`

.
Choosing ICA will execute the ordering procedure described in Trapnell and Cacchiarelli et al.,
which was implemented in Monocle version 1. `DDRTree`

is a more recent manifold
learning algorithm developed by Qi Mao and colleages. It is substantially more
powerful, accurate, and robust for single-cell trajectory analysis than ICA,
and is now the default method.

Often, experiments include cells from different batches or treatments. You can
reduce the effects of these treatments by transforming the data with a linear
model prior to dimensionality reduction. To do so, provide a model formula
through `residualModelFormulaStr`

.

Prior to reducing the dimensionality of the data, it usually helps
to normalize it so that highly expressed or highly variable genes don't
dominate the computation. `reduceDimension()`

automatically transforms
the data in one of several ways depending on the `expressionFamily`

of
the CellDataSet object. If the expressionFamily is `negbinomial`

or `negbinomial.size`

, the
data are variance-stabilized. If the expressionFamily is `Tobit`

, the data
are adjusted by adding a pseudocount (of 1 by default) and then log-transformed.
If you don't want any transformation at all, set norm_method to "none" and
pseudo_expr to 0. This maybe useful for single-cell qPCR data, or data you've
already transformed yourself in some way.

an updated CellDataSet object

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