dot-tm_complete_gr: Fill in a GRanges that carries sequences but not their...

In TmCalculator: Genome-Wide Nucleic Acid Melting Temperature Profiling and Multi-Omics Integration

Fill in a GRanges that carries sequences but not their complements

Description

Every other input form reaches to_genomic_ranges, which derives the complement. A GRanges handed straight to tm_calculate skipped that step, so an object built with a sequence column and nothing else (which is exactly what regions treats as a source) reached the compiled core with some sequences and no complements and failed there, on a message about cseqs that says nothing about what the caller did.

Usage

.tm_complete_gr(gr)

Arguments

gr	A GRanges.

Details

The complement is the plain base-for-base one, not the reverse complement: tm_nn() reads the two strands in register, so reversing one would pair every position with the wrong partner.

Value

The same object, with a complement column.

TmCalculator documentation built on Oct. 5, 2026, 5:08 p.m.