VIMiQ5_685: Amplification Reaction Using the Bio-Rad iQ5

In chipPCR: Toolkit of Helper Functions to Pre-Process Amplification Data

Description

Data set of an amplification reaction using the Bio-Rad iQ5 thermo cycler. The samples of Vimentin were amplified in the iQ5 as replicates according to Roediger et al. (2013). The quantification was performed during the elongation step (68.5 degrees Celsius).

Usage

data(VIMiQ5_685)

Format

A data frame with 40 observations on the following 97 variables. The first column ("Cycles") contains the number of cycles and consecutive columns contain the replicates ("A01" to "H12").

Source

Stefan Roediger, Claudia Deutschmann (BTU Cottbus - Senftenberg)

References

A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:33–74, 2013.

Examples

T595 <- rowMeans(VIMiQ5_595[, 2:ncol(VIMiQ5_595)])
T685 <- rowMeans(VIMiQ5_685[, 2:ncol(VIMiQ5_685)])

plot(1:length(T595), T595, main = "Fluorescence at different 
      temperatures\nQuantification in iQ5 (Bio-Rad)", xlab = "Cycle", 
      ylab = "Cycle dependent fluorescence", pch = 15, type = "b")
lines(1:length(T685), T685, pch = 19, type = "b", col = 2)
legend(1, 10000, c("Annealing (59.5 deg C)", "Elongation (68.5 deg C)"), 
      pch = c(15, 19), col = c(1,2))

chipPCR documentation built on March 5, 2021, 9:06 a.m.