VIMiQ5_melt: Melting Curve Measured with the Bio-Rad iQ5

In chipPCR: Toolkit of Helper Functions to Pre-Process Amplification Data

Description

Data set of a melting curve using the Bio-Rad iQ5 thermo cycler. The samples of Vimentin were measured in the CFX96 as replicates according to Roediger et al. (2013). The quantification was performed during the gradient from 55 to 95 degrees Celsius with a resolution of 0.5 degrees Celsius per step.

Usage

data(VIMiQ5_melt)

Format

A data frame with 81 observations on the following 97 variables. The first column ("T") contains the temperature and consecutive columns contain the replicates ("A01" to "H12").

Source

Stefan Roediger, Claudia Deutschmann (BTU Cottbus - Senftenberg)

References

A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:33–74, 2013.

Examples

data(VIMiQ5_melt)
tmp <- VIMiQ5_melt

plot(NA, NA, xlim = c(55,95), ylim = c(0, 40000), 
    xlab = "Temperature (degrees Celsius)",ylab = "RFU", 
    main = "Melting curve in iQ5 (Bio-Rad)")
apply(tmp[, 2:ncol(tmp)], 2, 
      function(x) lines(tmp[1:nrow(tmp),1],x))

Fmean <- rowMeans(tmp[, 2:ncol(tmp)])
lines(tmp[1:nrow(tmp),1], Fmean, col = "red", lwd = 3)

legend(55, 4000, c("Raw", "Mean"), pch = c(19,19), col = c(1,2))

Example output

NULL

chipPCR documentation built on March 5, 2021, 9:06 a.m.