View source: R/BunisHSPCData.R
BunisHSPCData | R Documentation |
Obtain the human fetal, newborn, and adult haematopoietic stem and progenitor cell single-cell RNA-seq dataset from Bunis et al. (2021).
BunisHSPCData(filtered = TRUE, legacy = FALSE)
filtered |
Logical scalar or "cells" indicating whether to:
|
legacy |
Logical scalar indicating whether to pull data from ExperimentHub. By default, we use data from the gypsum backend. |
Column metadata is recreated from GEO using the author-supplied TSV of per-cell annotations, or retrieved from a processed version of the data shared by authors via figshare. This contains information such as the tissue & sample of origin, age group, likely cell type, and Developmental Stage Scoring. Within DevStageScoring element of the column metadata are the applied results ('<cell_type>_scores') of random forest regression trained on the fetal (score = 0) and adult (score = 1) cells of individual cell types indicated by ('<cell_type>_inTraining').
If filtered=TRUE
, only the cells used by the authors in their final analysis are returned.
Otherwise, an additional retained
field will be present in the colData
, indicating whether the cell was retained by the authors.
All data are downloaded from ExperimentHub and cached for local re-use.
Specific resources can be retrieved by searching for scRNAseq/bunis-hspc
.
A SingleCellExperiment object with a single matrix of UMI counts.
Daniel Bunis
Bunis DG et al. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells Cell Rep. 34(1): 108573
sce <- BunisHSPCData()
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