View source: R/ErnstSpermatogenesisData.R
ErnstSpermatogenesisData | R Documentation |
Obtain the mouse spermatogenesis single-cell RNA-seq dataset from Ernst et al. (2019).
ErnstSpermatogenesisData(
method = c("emptyDrops", "Cellranger"),
location = TRUE,
legacy = FALSE
)
method |
String indicating which cell caller to obtain results for. |
location |
Logical scalar indicating whether genomic coordinates should be returned. |
legacy |
Logical scalar indicating whether to pull data from ExperimentHub. By default, we use data from the gypsum backend. |
This study contains two analyses done with datasets from different cell calling algorithms.
One uses Cellranger version 2 while the other uses emptyDrops
from DropletUtils.
Column metadata includes sample information, per-cell QC metrics and cell type labels. In particular, the sample label specifies the developmental stage of the mouse.
Note that method="Cellranger"
contains additional data for Tc1 mice.
These mice have an additional human chromosome 21 inserted alongside the usual mouse chromosomes.
If location=TRUE
, the coordinates of the Ensembl gene models are stored in the rowRanges
of the output.
All data are downloaded from ExperimentHub and cached for local re-use.
Specific resources can be retrieved by searching for scRNAseq/ernst-spermatogenesis
.
A SingleCellExperiment object with a single matrix of UMI counts.
Aaron Lun
Ernst C et al. (2019). Staged developmental mapping and X chromosome transcriptional dynamics during mouse spermatogenesis. Nat Commun 10, 1251
if (.Machine$sizeof.pointer > 4) { # too large for 32-bit machines!
sce <- ErnstSpermatogenesisData()
}
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