PollenGliaData: Obtain the Pollen radial glia data

In LTLA/scRNAseq: Collection of Public Single-Cell RNA-Seq Datasets

Obtain the Pollen radial glia data

Description

Obtain the human radial glia single-cell RNA-seq dataset from Pollen et al. (2017).

Usage

PollenGliaData(ensembl = FALSE, location = TRUE, legacy = FALSE)

Arguments

ensembl	Logical scalar indicating whether the output row names should contain Ensembl identifiers.
location	Logical scalar indicating whether genomic coordinates should be returned.
legacy	Logical scalar indicating whether to pull data from ExperimentHub. By default, we use data from the gypsum backend.

Details

Column metadata includes the anatomical source, sample of origin, presumed cell type and assorted alignment statistics.

If ensembl=TRUE, the gene symbols are converted to Ensembl IDs in the row names of the output object. Rows with missing Ensembl IDs are discarded, and only the first occurrence of duplicated IDs is retained.

If location=TRUE, the coordinates of the Ensembl gene models are stored in the rowRanges of the output. This is only performed when ensembl=TRUE.

All data are downloaded from ExperimentHub and cached for local re-use. Specific resources can be retrieved by searching for scRNAseq/pollen-glia.

Value

A SingleCellExperiment object with a single matrix of read counts.

Author(s)

Aaron Lun

References

Pollen A et al. (2017). Molecular identity of human outer radial glia during cortical development. Cell 163, 55-67.

Examples

sce <- PollenGliaData()

LTLA/scRNAseq documentation built on April 24, 2024, 5:58 p.m.