View source: R/KolodziejczykESCData.R
KolodziejczykESCData | R Documentation |
Obtain the mouse embryonic stem cell single-cell RNA-seq data from Kolodziejczyk et al. (2015).
KolodziejczykESCData(remove.htseq = TRUE, location = TRUE, legacy = FALSE)
remove.htseq |
Logical scalar indicating whether HT-seq alignment statistics should be removed. |
location |
Logical scalar indicating whether genomic coordinates should be returned. |
legacy |
Logical scalar indicating whether to pull data from ExperimentHub. By default, we use data from the gypsum backend. |
Column metadata is generated from the column names, and contains the culture conditions and the plate of origin for each cell.
Count data for ERCC spike-ins are stored in the "ERCC"
entry in the altExps
.
If location=TRUE
, the coordinates of the Ensembl gene models are stored in the rowRanges
of the output.
All data are downloaded from ExperimentHub and cached for local re-use.
Specific resources can be retrieved by searching for scRNAseq/kolodziejczyk-esc
.
A SingleCellExperiment object with a single matrix of read counts.
Aaron Lun
Messmer T et al. (2019). Transcriptional heterogeneity in naive and primed human pluripotent stem cells at single-cell resolution. Cell Rep 26(4), 815-824.e4
sce <- KolodziejczykESCData()
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