View source: R/ERCCSpikeInConcentrations.R
ERCCSpikeInConcentrations | R Documentation |
Obtain ERCC spike-in concentrations from the Thermo Fisher Scientific website.
ERCCSpikeInConcentrations(
volume = NULL,
dilution = NULL,
mix = c("1", "2"),
legacy = FALSE
)
volume |
Numeric scalar specifying the added volume (in nanoliters) of ERCC spike-in mixture.
Only used if |
dilution |
Numeric scalar specifying the dilution factor used for the added volume of the spike-in mixture.
Only used if |
mix |
String specifying whether to compute the number of molecules for mix 1 or 2.
Only used if both |
legacy |
Logical scalar indicating whether to pull data from ExperimentHub. By default, we use data from the gypsum backend. |
If volume
and dilution
are specified,
an additional column is added to the output specifying the number of molecules of spike-in transcipt for the specified mix.
All data are downloaded from ExperimentHub and cached for local re-use.
Specific resources can be retrieved by searching for scRNAseq/ercc-concentrations
.
A DataFrame object with one row per ERCC spike-in transcript. This contains information such as the spike-in concentration in each mix.
Alan O'Callaghan
df <- ERCCSpikeInConcentrations()
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