View source: R/countErccMolecules.R
countErccMolecules | R Documentation |
Compute the number of molecules for each transcript in the ERCC spike-in mixture, based on their published concentration as well as the volume of the diluted mixture added to each cell.
countErccMolecules(volume, dilution, mix = c("1", "2"), ...)
volume |
Numeric scalar specifying the added volume (in microliters) of ERCC spike-in mixture. |
dilution |
Numeric scalar specifying the dilution factor used for the added volume of the spike-in mixture. |
mix |
String specifying whether to compute the number of molecules for mix 1 or 2. |
... |
Further arguments to pass to |
A DataFrame object with one row per ERCC spike-in transcript. This contains the estimated concentration and molecule count for each transcript.
Aaron Lun, based on code from Alan O'Callaghan
countErccMolecules(volume = 9, dilution = 300000)
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