Obtain the mouse embryonic stem cell single-cell RNA-seq data from Buettner et al. (2015).
Logical scalar indicating whether HT-seq alignment statistics should be removed.
Rows corresponding to HT-seq's alignment statistics are removed by default.
These can be retained by setting
Column metadata contains the experimentally determined cell cycle phase for each cell.
Counts for ERCC spike-ins are stored in the
"ERCC" entry in the
All data are downloaded from ExperimentHub and cached for local re-use.
Specific resources can be retrieved by searching for
A SingleCellExperiment object with a single matrix of read counts.
Buettner F et al. (2015). Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells. Nat. Biotechnol. 33(2), 155-160.
sce <- BuettnerESCData()
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