R/BuettnerESCData.R

Defines functions BuettnerESCData

Documented in BuettnerESCData

#' Obtain the Buettner ESC data
#'
#' Obtain the mouse embryonic stem cell single-cell RNA-seq data from Buettner et al. (2015).
#'
#' @param remove.htseq Logical scalar indicating whether HT-seq alignment statistics should be removed.
#' @param location Logical scalar indicating whether genomic coordinates should be returned.
#' @param legacy Logical scalar indicating whether to pull data from ExperimentHub.
#' By default, we use data from the gypsum backend.
#'
#' @details
#' Rows corresponding to HT-seq's alignment statistics are removed by default.
#' These can be retained by setting \code{remove.htseq=FALSE}.
#'
#' Column metadata contains the experimentally determined cell cycle phase for each cell.
#'
#' Counts for ERCC spike-ins are stored in the \code{"ERCC"} entry in the \code{\link{altExps}}.
#'
#' If \code{location=TRUE}, the coordinates of the Ensembl gene models are stored in the \code{\link{rowRanges}} of the output.
#'
#' All data are downloaded from ExperimentHub and cached for local re-use.
#' Specific resources can be retrieved by searching for \code{scRNAseq/buettner-esc}.
#'
#' @return A \linkS4class{SingleCellExperiment} object with a single matrix of read counts.
#'
#' @author Aaron Lun
#'
#' @references
#' Buettner F et al. (2015).
#' Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells.
#' \emph{Nat. Biotechnol.} 33(2), 155-160.
#' 
#' @examples
#' sce <- BuettnerESCData()
#' 
#' @export
#' @importFrom SingleCellExperiment splitAltExps 
BuettnerESCData <- function(remove.htseq=TRUE, location=TRUE, legacy=FALSE) {
    if (!legacy) {
        sce <- fetchDataset("buettner-esc-2015", "2024-04-18", realize.assays=TRUE)

    } else {
        version <- "2.0.0"
        sce <- .create_sce(file.path("buettner-esc", version), has.rowdata=TRUE)

        if (remove.htseq) {
            sce <- sce[grepl("^ENSMUS", rownames(sce)) | grepl("^ERCC", rownames(sce)),]
        }

        status <- ifelse(grepl("^ERCC-[0-9]+", rownames(sce)), "ERCC", "endogenous")
        sce <- splitAltExps(sce, status, ref="endogenous")
        spike.exp <- altExp(sce, "ERCC")
        spikedata <- ERCCSpikeInConcentrations(volume = 1, dilution = 1000)
        spikedata <- spikedata[rownames(spike.exp),]

        rowData(spike.exp) <- cbind(rowData(spike.exp), spikedata)
        rowData(spike.exp)$featureType <- NULL # redundant field; what else would it be!?
        altExp(sce, "ERCC") <- spike.exp
    }

    .define_location_from_ensembl(sce, species="Mm", location=location)
}
LTLA/scRNAseq documentation built on June 28, 2024, 7:31 p.m.