Description Usage Arguments Value
生成各个marker的tSNE;
生成各个marker的tSNE;
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 | draw_tsne_heatmaps(
combined_data_plot,
heatmap_tsne_markers = NULL,
trans_method = "simpleAsinh",
single_file = FALSE,
groups = groups,
major_cond = NULL,
dot_size = 4,
dot_colours = colorRampPalette(c("#00007F", "blue", "#007FFF", "cyan", "#7FFF7F",
"yellow", "#FF7F00", "red", "#7F0000")),
show_legend = TRUE,
show_scale = TRUE,
output_dir = "tsne_heatmap",
output_format = "tiff",
reduction_dm1 = "tsne_1",
reduction_dm2 = "tsne_2"
)
draw_tsne_heatmaps(
combined_data_plot,
heatmap_tsne_markers = NULL,
trans_method = "simpleAsinh",
single_file = FALSE,
groups = groups,
major_cond = NULL,
dot_size = 4,
dot_colours = colorRampPalette(c("#00007F", "blue", "#007FFF", "cyan", "#7FFF7F",
"yellow", "#FF7F00", "red", "#7F0000")),
show_legend = TRUE,
show_scale = TRUE,
output_dir = "tsne_heatmap",
output_format = "tiff",
reduction_dm1 = "tsne_1",
reduction_dm2 = "tsne_2"
)
|
combined_data_plot |
数据框或者矩阵,附带有(meta)cluster、降维结果(t_sne_1,t_sne_2)的表达矩阵 |
heatmap_tsne_markers |
vector,指定marker的名称,默认NULL,此时将生成所有通道的heatmap |
trans_method |
数据转化方式,有四种:"CytofAsinh","simpleAsinh";"0_to_Max",所有Marker的信号强度除以最大值,线性转换,通过除以各通道信号的最大值把数值scale到0~1;"Min_to_Max",线性转换,最小值转换成0,最大值转换成1,最大限度展现population的表达差异 |
single_file |
是否把所有heatmap合成单个文件,默认FALSE |
groups |
实验组的Metadata信息,其中必需包含列名:”Short_name“ |
major_cond |
选择groups中一个列名做为展现差异的major_cond |
dot_size |
图中点的大小,默认是4 |
dot_colours |
设置colorbar的颜色,默认为jet color函数 |
show_legend |
是否显示legend(colorbar) |
show_scale |
是否显示X 轴和Y轴的刻度,默认是TRUE |
output_dir |
输出数据文件夹名称,默认是TRUE |
output_format |
输出数据文件格式:"tiff"和“pdf” |
reduction_dm1 |
combined_data_plot降维产生的维度,默认"tsne_1" |
reduction_dm2 |
combined_data_plot降维产生的维度,默认"tsne_2" |
edge_data |
画网络图的时候,存储网络连接数据 |
groups_to_show |
vector,指定在图上显示哪些组的数据(组名应属于all_samples的major_cond列),默认为NULL,显示所有组 |
legend_type |
legend(colorbar)是局部("local"(默认)),所有marker用不同的colorbar)还是全局("global",所有marker共用一个colorbar) |
legend_limit |
仅在legend_type是"global"的情况下有效,手动设置colorbar的上下限,取值为一个vector,例如c(0,5) 0为最小值,5为最大值 |
show_axis |
是否显示坐标轴,默认是TRUE |
none
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