AcupFromRSA: Convert RSA to Acup

In mooredf22/protlocassign: Estimation of protein distribution from quantitative subcellular fractionation experiments using a constrained proportional assignment model

Convert RSA to Acup

Description

Convert RSA (relative specific amount) profiles to Acup (relative amount) profiles

Usage

AcupFromRSA(RSA, NstartMaterialFractions, totProt = NULL)

Arguments

RSA	matrix of relative specific amount profiles (see help file for protRSA_test)
NstartMaterialFractions	number of fractions that reconstitute the starting material, e.g., a complete set of differential centrifugation fractions. For experiment AT5, it is 6 ( N, M, L1, L2, P, and S).
totProt	vector of total protein amounts (derived from a given amount of starting material) in each of the fractions comprising the profile

Value

Acup profiles: amount of given protein in fraction / amount of that given protein in starting material (see help file protAcup_test).

Examples

data(protRSA_test)
data(totProtAT5)
protAcup_out2 <- AcupFromRSA(protRSA_test[,seq_len(9)],
            NstartMaterialFractions = 6, totProt=totProtAT5)
round(head(protAcup_out2), digits=4)
str(protAcup_out2)

mooredf22/protlocassign documentation built on Sept. 13, 2023, 3:57 p.m.