NSAfromAcup: Convert Acup to NSA

In mooredf22/protlocassign: Estimation of protein distribution from quantitative subcellular fractionation experiments using a constrained proportional assignment model

Convert Acup to NSA

Description

Convert Acup (relative amount) profiles to NSA (normalized specific amount) profiles

Usage

NSAfromAcup(Acup, NstartMaterialFractions, totProt = NULL)

Arguments

Acup	data frame containing Acup profiles; amount of given protein in fraction / amount of that given protein in starting material (see help file for protAcup_test).
NstartMaterialFractions	number of fractions that reconstitute the starting material, e.g., a complete set of differential centrifugation fractions. For experiment AT5, it is 6 ( N, M, L1, L2, P, and S).
totProt	vector of total protein amounts (derived from a given amount of starting material) in each of the fractions comprising the profile

Value

A data frame of NSA profiles (see help file for protNSA_test)

Examples

data(protAcup_test)
data(totProtAT5)
protNSA_out1 <- NSAfromAcup(protAcup_test[,seq_len(9)], 
            NstartMaterialFractions = 6,
            totProt=totProtAT5)
round(head(protNSA_out1), digits=4)
str(protNSA_out1)

mooredf22/protlocassign documentation built on Sept. 13, 2023, 3:57 p.m.