NSAfromRSA: Convert RSA to NSA

In mooredf22/protlocassign: Estimation of protein distribution from quantitative subcellular fractionation experiments using a constrained proportional assignment model

Convert RSA to NSA

Description

Convert RSA (relative specific amount) profiles to NSA (normalized specific amount) profiles

Usage

NSAfromRSA(RSA)

Arguments

RSA

data frame containing RSA profiles. RSA is the ratio of two ratios: the numerator is the amount of a given protein in a particular fraction divided by the amount of that given protein in the starting material while the denominator is amount of total protein in a particular fraction divided by the amount of total protein in the starting material. The RSA describes the fold-enrichment (RSA>1) or depletion (RSA<1) of a protein during the fractionation process, and is analogous to the relative specific activity term used in classical analytical subcellular fractionation.

Value

A data frame of NSA profiles (see help file for protNSA_test)

Examples

data(protRSA_test)
protNSA_out2 <- NSAfromRSA(protRSA_test[,seq_len(9)])
round(head(protNSA_out2), digits=4)
str(protNSA_out2)

mooredf22/protlocassign documentation built on Sept. 13, 2023, 3:57 p.m.