trichelab/spiky: Spike-in calibration for cell-free MeDIP

spiky implements methods and model generation for cfMeDIP (cell-free methylated DNA immunoprecipitation) with spike-in controls. CfMeDIP is an enrichment protocol which avoids destructive conversion of scarce template, making it ideal as a "liquid biopsy," but creating certain challenges in comparing results across specimens, subjects, and experiments. The use of synthetic spike-in standard oligos allows diagnostics performed with cfMeDIP to quantitatively compare samples across subjects, experiments, and time points in both relative and absolute terms.

Getting started

Package details

Bioconductor views DNAMethylation DifferentialMethylation Normalization Preprocessing QualityControl Sequencing
Package repositoryView on GitHub
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trichelab/spiky documentation built on Sept. 17, 2022, 8:44 a.m.