FOP: Main function to find the positivity for either tissue, cell...

In AstroPathJHU/mIFTO: mIFTO: Multiplex Immunoflourescence Titration Optimization

Main function to find the positivity for either tissue, cell segmentations and percent positive pixels

Description

FOP Created By: Benjamin Green Last Edited 07/15/2020

Usage

FOP()

Details

FOP this code will find positivity for either tissue seg, cell seg, or PPC pixel data## Created By: Benjamin Green 07.12.2018 to run: 1. Organize data into seperate folders according to conditions 2. Load the Titration Package 3. Type FOP and ENTER 4. Enter the inofrmation to the prompt Slide descriptor is the description of slide (ie Tonsil1, Tonsil3) Other condition delineation is something else that delinates conditions (ie monoplex/multplex, V1/ V2, 1to50/1to100) this does not need to be in the name of the slide just to differentiate conditions 5.Click Run then select directory with the data 6.A new yes/no box will open this is for additional conditions/ AB with the same Slide Descriptors if there are more click yes if not click no 7. for yes: follow on screen prompts till there are no more coniditions 8. for no: a browsing window will open; select where you want output table to go

Value

the output is a csv file that has the following columns: concentration, one for each slide descriptors, and Antibody

AstroPathJHU/mIFTO documentation built on April 14, 2025, 7:22 a.m.