thresholding.medians: Used by analysis functions to calculate the median signal to...

In AstroPathJHU/mIFTO: mIFTO: Multiplex Immunoflourescence Titration Optimization

Used by analysis functions to calculate the median signal to noise ratios

Description

thresholding.medians Created By: Benjamin Green Last Edited 11/12/2018

Usage

thresholding.medians(
  data.in,
  threshold,
  Slide.ID,
  Concentration,
  connected.pixels
)

Arguments

data.in	a single column data table with the values of interest
threshold	a single value to divide the population, everything equal to and above is positive
Slide.ID	a slide distinguisher
Concentration	is the concentration of the choosen AB

Details

This function divides the given data at the threshold given and calculates a median for the above and below values The function then calculates and returns a signal to noise calculation

Value

a data.frame with Signal, Noise, S/N, Slide.ID, Concentration

AstroPathJHU/mIFTO documentation built on April 14, 2025, 7:22 a.m.