ZeiselBrainData: Obtain the Zeisel brain data

In LTLA/scRNAseq: Collection of Public Single-Cell RNA-Seq Datasets

Obtain the Zeisel brain data

Description

Obtain the mouse brain single-cell RNA-seq dataset from Zeisel et al. (2015).

Usage

ZeiselBrainData(ensembl = FALSE, location = TRUE, legacy = FALSE)

Arguments

ensembl	Logical scalar indicating whether the output row names should contain Ensembl identifiers.
location	Logical scalar indicating whether genomic coordinates should be returned.
legacy	Logical scalar indicating whether to pull data from ExperimentHub. By default, we use data from the gypsum backend.

Details

Row data contains a single "featureType" field describing the type of each feature (endogenous genes, mitochondrial genes, spike-in transcripts and repeats). Spike-ins and repeats are stored as separate entries in the altExps.

Column metadata is provided in the same form as supplied in http://linnarssonlab.org/cortex/. This contains information such as the cell diameter and the published cell type annotations.

If ensembl=TRUE, the gene symbols are converted to Ensembl IDs in the row names of the output object. Rows with missing Ensembl IDs are discarded, and only the first occurrence of duplicated IDs is retained.

If location=TRUE, the coordinates of the Ensembl gene models are stored in the rowRanges of the output.

Spike-in metadata is added using ERCCSpikeInConcentrations, with molecule counts computed using a volume of 9 nL per cell at a dilution of 1:20000.

All data are downloaded from ExperimentHub and cached for local re-use. Specific resources can be retrieved by searching for scRNAseq/zeisel-brain.

Value

A SingleCellExperiment object with a single matrix of UMI counts.

Author(s)

Aaron Lun

References

Zeisel A et al. (2015). Brain structure. Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq. Science 347(6226), 1138-42.

Examples

sce <- ZeiselBrainData()

LTLA/scRNAseq documentation built on June 28, 2024, 7:31 p.m.