#' Profiles the nucleotide content of intervals in a fasta file.
#'
#' @param fi <fasta>
#' @param bed <bed/gff/vcf>
#' @param s Profile the sequence according to strand.
#'
#' @param seq Print the extracted sequence
#'
#' @param pattern Report the number of times a user-defined sequence
#' is observed (case-sensitive).
#'
#' @param C Ignore case when matching -pattern. By defaulty, case matters.
#'
#' @param fullHeader Use full fasta header.
#' - By default, only the word before the first space or tab is used.
#'
#' @param output Output filepath instead of returning output in R.
#'
bt.nuc <- function(fi, bed, s = NULL, seq = NULL, pattern = NULL, C = NULL, fullHeader = NULL, output = NULL)
{
# Required Inputs
fi <- establishPaths(input=fi, name="fi", allowRobjects=TRUE)
bed <- establishPaths(input=bed, name="bed", allowRobjects=TRUE)
options <- ""
# Options
options <- createOptions(names=c("s", "seq", "pattern", "C", "fullHeader"), values=list(s, seq, pattern, C, fullHeader))
# establish output file
tempfile <- tempfile("bedtoolsr", fileext=".txt")
tempfile <- gsub("\\", "/", tempfile, fixed=TRUE)
bedtools.path <- getOption("bedtools.path")
if(!is.null(bedtools.path)) bedtools.path <- paste0(bedtools.path, "/")
cmd <- paste0(bedtools.path, "bedtools nuc ", options, " -fi ", fi[[1]], " -bed ", bed[[1]], " > ", tempfile)
if(.Platform$OS.type == "windows") shell(cmd) else system(cmd)
if(!is.null(output)) {
if(file.info(tempfile)$size > 0)
file.copy(tempfile, output)
} else {
if(file.info(tempfile)$size > 0)
results <- utils::read.table(tempfile, header=FALSE, sep="\t", quote='')
else
results <- data.frame()
}
# Delete temp files
temp.files <- c(tempfile, fi[[2]], bed[[2]])
deleteTempFiles(temp.files)
if(is.null(output))
return(results)
}
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