cubeCLEAVRMF: Inheritance Cube: CLEAVR - Cleave and Rescue

In MGDrivE: Mosquito Gene Drive Explorer

Inheritance Cube: CLEAVR - Cleave and Rescue

Description

This is a novel cube from the Akbari lab. There are 2 loci: the first is a female fertility locus (e.g. doubleSex), where the Cas, gRNAs, and a recoded essential gene go. This locus is inherited in a Mendelian fashion, but is also targeted for destruction by the homing allele. The second locus involves an essential gene, for both males and females, and this is the target of the gRNAs at the first locus. No homing is performed, it is simply destroyed. There is different cutting rates in males and females, with no possibility for a rescuing resistant allele. Females homozygous for the H or B alleles at locus 1 are viable but infertile, while males are unaffected. All animals homozygous at locus two must contain the recoded copy at locus 1 to be viable. This version corresponds to the homing construct being autosomal.

Usage

cubeCLEAVRMF(
  cM1 = 1,
  cM2 = 1,
  cF1 = 1,
  cF2 = 1,
  eta = NULL,
  phi = NULL,
  omega = NULL,
  xiF = NULL,
  xiM = NULL,
  s = NULL
)

Arguments

cM1	Male cutting rate at first locus
cM2	Male cutting rate at second locus
cF1	Female cutting rate at first locus
cF2	Female cutting rate at second locus
eta	Genotype-specific mating fitness
phi	Genotype-specific sex ratio at emergence
omega	Genotype-specific multiplicative modifier of adult mortality
xiF	Genotype-specific female pupatory success
xiM	Genotype-specific male pupatory success
s	Genotype-specific fractional reduction(increase) in fertility

Value

Named list containing the inheritance cube, transition matrix, genotypes, wild-type allele, and all genotype-specific parameters.

MGDrivE documentation built on Sept. 9, 2025, 5:42 p.m.