cubeSplitDriveX: Inheritance Cube: X-linked Split CRISPR Drive with 2...

In MGDrivE: Mosquito Gene Drive Explorer

Inheritance Cube: X-linked Split CRISPR Drive with 2 Resistance Alleles and male/female specific homing

Description

This is a X-linked, sex-specific version of a split CRISPR drive. At the X locus is the Cas9, inherited in a Mendelian fashion. At a second, unlinked, autosomal locus are the gRNAs. When the two loci occur together, the gRNAs drive, with potential damaged alleles, but the Cas9 remains Mendelian. Deposition in this cube is performed when both pieces come together in females. This drive has 2 loci:

• "Locus" 1, sex chromosomes, has 3 alleles

  • X: Wild-type X chromosome

  • C: X-chromosome carrying a Cas9 construct

  • Y: Wild-type Y chromosome

• Locus 2, autosomal locus, has 4 alleles:

  • W: Wild-type allele

  • G: gRNA allele

  • R: Functional or low-cost resistance allele

  • B: Non-functional or high-cost resistance allele

Usage

cubeSplitDriveX(
  cM = 1,
  chM = 0,
  crM = 0,
  cF = 1,
  chF = 0,
  crF = 0,
  ccF = cF,
  cchF = chF,
  ccrF = crF,
  dW = 0,
  dhW = 0,
  drW = 0,
  ddW = dW,
  ddhW = dhW,
  ddrW = drW,
  eta = NULL,
  phi = NULL,
  omega = NULL,
  xiF = NULL,
  xiM = NULL,
  s = NULL
)

Arguments

cM	Cutting efficiency in males
chM	Homing efficiency in males
crM	Resistance efficiency in males
cF	Cutting efficiency in females, one Cas9 allele
chF	Homing efficiency in females, one Cas9 allele
crF	Resistance efficiency in females, one Cas9 allele
ccF	Cutting efficiency in females, two Cas9 alleles
cchF	Homing efficiency in females, two Cas9 alleles
ccrF	Resistance efficiency in females, two Cas9 alleles
dW	Maternal deposition cutting, one Cas9 allele
dhW	Maternal deposition homing, one Cas9 allele
drW	Maternal deposition resistance, one Cas9 allele
ddW	Maternal deposition cutting, two Cas9 alleles
ddhW	Maternal deposition homing, two Cas9 alleles
ddrW	Maternal deposition resistance, two Cas9 alleles
eta	Genotype-specific mating fitness
phi	Genotype-specific sex ratio at emergence
omega	Genotype-specific multiplicative modifier of adult mortality
xiF	Genotype-specific female pupatory success
xiM	Genotype-specific male pupatory success
s	Genotype-specific fractional reduction(increase) in fertility

Value

Named list containing the inheritance cube, transition matrix, genotypes, wild-type allele, and all genotype-specific parameters.

MGDrivE documentation built on Sept. 9, 2025, 5:42 p.m.