NormalizeExpression: Normalizes gene expression in the PISCESgexp assay. Stores...

View source: R/object_funcs.R

NormalizeExpressionR Documentation

Normalizes gene expression in the PISCESgexp assay. Stores normalized counts in 'data' field. Additionally, generates a scaled matrix (interpretable as an internal GES) to the 'scale.data' field.

Description

Normalizes gene expression in the PISCESgexp assay. Stores normalized counts in 'data' field. Additionally, generates a scaled matrix (interpretable as an internal GES) to the 'scale.data' field.

Usage

NormalizeExpression(pisces.obj, method = c("cpm", "pflogpf"), filt.per = 0.01)

Arguments

pisces.obj

A Seurat object with the PISCESgexp assay.

method

One of 'cpm' or 'pflogpf'

filt.per

Threshold value for gene filtration; genes w/ less than filt.per * num.samps total counts will be removed. Default of 0.01.

Value

A Seurat object with normalized expression in the 'data' field of the PISCESgexp assay.


califano-lab/PISCES documentation built on Jan. 11, 2023, 5:34 a.m.