Man pages for combiz/scFlow
A Single-Cell/Nuclei Analysis Toolkit

annotate_celltype_metricsAnnotate a SingleCellExperiment With Cell-type Metrics
annotate_integrated_sceAnnotate integrated, reduced dimension, and clustered...
annotate_merged_sceAnnotate a post-merge SingleCellExperiment with plots
annotate_sceAnnotate a SingleCellExperiment With Gene Names and QC...
annotate_sce_cellsAdd basic cell-wise annotations for a SingleCellExperiment
annotate_sce_genesAdd basic gene-wise annotations for a SingleCellExperiment
cluster_sceCluster SingleCellExperiment with monocle3::cluster_cells
dot-append_cell_metric_plots_sceAttach celltype associated metric plots to sce
dot-append_cell_prop_plots_sceAttach proportional celltype plots to sce
dot-append_celltype_plots_sceHelper function to plot celltypes in reduced dimensions
dot-append_citation_sceAppend a citation to a SingleCellExperiment
dot-barplot_gseabarplot for GSEA. x axis normalizedEnrichmentScore, y axis...
dot-calculate_retain_parameterhelper fn - calculate the retain parameter for emptydrops...
dot-check_modelcheck model is full rank
dot-check_sce_for_ligerHelper function to check SCE before running liger
dot-clean_ggplot_plot_envRemove objects from a ggplot plot_env
dot-clean_ggplot_quosuresRemove unwanted env variables from ggplot quosures
dot-clean_quosure_envRemove unwanted variables from a quosure environment
dot-dotplot_enrichrdotplot for ORA. x axis perturbation, y axis description
dot-dotplot_oradotplot for ORA. x axis enrichment_ratio, y axis description
dot-doublet_finder_plot_dim_redplot 2d dimensionality reduction with is_doublet colour
dot-doublet_finder_plot_param_sweeppparam sweep plot, pK vs BCmetricx with BCmetric maxima...
dot-expand_sce_rowsHelper function to expand a SingleCellExperiment to include...
dot-fancy_scientificscale_y_continuous(labels=.fancy_scientific)
dot-filter_sce_genes_for_deSubset a SingleCellExperiment for genes meeting expressivity...
dot-format_res_table_enrichrFormat result table
dot-format_res_table_ORAFormat result table
dot-formula_to_charmodel formula to char/string
dot-generate_merge_summary_plothelper fn to generate a merge summary plot for a...
dot-generate_model_from_varsGenerate a model formula from dependent, confounding, and...
dot-generate_pbsce_by_idhelper fn to generate a pseudobulk sce for post-merge qc
dot-get_d_paletteRetrieve a palette of n_colours discrete colours from...
dot-get_variance_explainedGet the variance explained by a variable for all genes
dot-get_x_most_specific_genesFind x most specific genes
dot-grobify_ggplotClean ggplot2 env's by generating a ggplot2 grob then...
dot-map_celltypesMain mapping function
dot-map_celltypes_with_ewceMap cluster celltypes with EWCE
dot-model_fisher_celltypeRun a fisher exact test on absolute cell numbers for each...
dot-perform_de_with_mastPerform DE with MAST
dot-perform_ks_uniformity_testhelper fn - Kolmogorov-Smirnov Uniformity Test
dot-plot_dirichlet_resultsPlot relative celltype proportions with Dirichlet model...
dot-plot_emptydrops_distributionhelper fn - histogram plot to confirm uniform distribution
dot-plot_emptydrops_loglikehelper fn - histogram plot to confirm uniform distribution
dot-plot_heatmap_of_pbscehelper fn to generate a heatmap and dendrogram for features...
dot-plot_n_cells_by_unique_id_varhelper fn to generate a cell numbers plot by sample
dot-plot_unique_id_varPlot relative celltype proportions by sample
dot-prepare_dirichlet_plot_tablePrepare data for '.plot_dirichlet_results'
dot-prepare_fisher_counts_tablePrepare data for '.model_fisher_celltype'
dot-prepare_unique_id_var_plot_tablePrepare data
dot-preprocess_sce_for_dePreprocess the SCE for DE analysis
dot-preprocess_seurat_objecttemp fn
dot-print_citationPrint a single citation to stdout
dot-print_citation_htmlPrint a single citation to stdout as html
dot-process_dirichlet_fitProcess the fit model to generate an accessible summary...
dot-qc_append_summary_tablegenerate table of qc results
dot-qc_plot_count_depth_distributionx axis barcode rank, y axis total counts
dot-qc_plot_count_depth_histogramx axis count depth, y axis number of genes
dot-qc_plot_features_vs_count_depthx axis count depth, y axis number of genes
dot-qc_plot_mito_fraction_histogramx axis count depth, y axis number of genes
dot-qc_plot_number_genes_histogramx axis count depth, y axis number of genes
dot-qc_plot_ribo_fraction_histogramx axis count depth, y axis number of genes
dot-read_rds_files_to_listHelper function to read rds files into a list
dot-retrieve_covariatesRetrieve the covariates from a SCE colData
dot-sce_to_cdsConvert a SingleCellExperiment object o a CellDataSet...
dot-tally_cellsTally the cells in each celltype for each unique sample
dot-uniftestCopy of archived uniftest::kolmogorov.unif.test
dot-with_envRun function f in environment e
filter_sceFilter SingleCellExperiment according to QC metrics Quality...
find_cellsAnnotate a SingleCellExperiment with EmptyDrops predictions
find_impacted_pathwaysFunctional enrichment analysis
find_marker_genesFind specific markers for groups of cells
find_singletsFind singlets in a SingleCellExperiment
generate_sceGenerate a SingleCellExperiment object from a matrix and...
integrate_sceIntegrate datasets within a SingleCellExperiment Object
liger_preprocessPreprocessing steps for Liger dimensionality reduction
liger_reduce_dimsDimensionality reduction using Liger factorization
list_databasesCheck available databases and their alias for scFlow
map_celltypes_sceMap cluster celltypes with EWCE
map_custom_celltypesMap Custom Celltype Annotations from a DataFrame
map_ensembl_gene_idReturn mappings for Ensembl Gene IDs
merge_sceMerge Multiple SingleCellExperiment Objects
model_celltype_freqsModel Changes in Celltype Frequencies with Dirichlet...
pathway_analysis_enrichrFunctional enrichment analysis using enrichR
pathway_analysis_webgestaltrFunctional enrichment analysis using WebgestaltR
perform_dePerform Differential Gene Expression on a...
plot_expr_by_numeric_varPlot Gene Expression for Samples against Numerical Variable
plot_reduced_dimPlot Cells Reduced Dimensions With Feature Highlighting
plot_reduced_dim_genePlot Cells Reduced Dimensions With Gene Expression
plot_violinPlot Gene Expression Violin Plots Stratified by Group
pseudobulk_sceGenerate a Pseudo-bulk SingleCellExperiment from a...
read_celltype_mappingsRead Celltype Mappings
read_metadataRead the metadata for a sample from a samplesheet
read_sceRead a SingleCellExperiment from a folder
read_sparse_matrixRead a feature-barcode matrix
reduce_dims_sceCalculate dimensionality reductions for a...
report_celltype_metricsGenerate A Report of Cell-Type Metrics for a...
report_celltype_modelGenerate a report for a celltype proportion analysis
report_deGenerate a report for differential expression analysis
report_impacted_pathwayGenerate a report for impacted pathway analysis
report_integrated_sceGenerate a report for dataset integration, dims reduction,...
report_merged_sceGenerate plots and a QC report for a SingleCellExperiment
report_qc_sceGenerate plots and a QC report for a SingleCellExperiment
run_doubletfinderFind singlets in a SingleCellExperiment with DoubletFinder by...
sce_to_seutemp fn
volcano_plotPlot volcano plot for differential expression analysis
write_celltype_mappingsWrite Celltype Mappings
write_sceWrite a SingleCellExperiment to a folder
write_sparse_matrixWrite a feature-barcode matrix
combiz/scFlow documentation built on Feb. 25, 2024, 10:25 a.m.